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(A-B) Intracellular ROS productions of unstimulated cells (NO TREAT) or M1-polarized macrophages, treated with PBS (CON) or melittin (MEL) were measured by H2DCFDA staining respectively. (C) Relative phagocytosis index of melittin or cytochalashin D (cytoD)-treated BMDMs compared with control was estimated by measuring internalized latex-bead fluorescence intensity. The mean ± SEM for three replicates are shown. *P < 0.05, ***P < 0.0001.
