Skip to main content
. 2017 Jul 5;8(33):55003–55021. doi: 10.18632/oncotarget.18991

Figure 3. Cell cycle progression of cobalt complex 2 in HeLa cancer cells.

Figure 3

Exponentially growing HeLa cells were synchronized in the serum-free medium for 24 h. Then the cells were incubated with the DMSO and indicated concentrations of cobalt complex 2 for 24 h. (A) The cell cycle progression was evaluated using propidium iodide (PE-A) staining and flow cytometry analysis. The bar chart indicated the results of quantitative analysis of cell-cycle distribution (% of cell population). Means ± S.D. were from three independent experiments (One-way ANOVA: **P < 0.01, and ***P < 0.001). (B) Cobalt complex 2 altered the S phase specific cell cycle markers expression in HeLa cancer cells. HeLa cells were treated with DMSO or indicated concentrations of cobalt complex 2 for 24 h. Cell lysates were harvested and analyzed by Western blot for Cyclin D, Cyclin-dependent kinase 4 (Cdk4), Cyclin E, Cyclin-dependent kinase 2 (Cdk2), Cyclin A, E2F transcription factor, and β-actin.