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. 2017 Jul 8;8(33):55147–55161. doi: 10.18632/oncotarget.19101

Figure 3. Ac-LA inhibits the Akt signaling pathway and the release of angiogenic VEGF in prostate cancer cells.

Figure 3

(A) Ac-LA rapidly inhibits the Akt, but not the mTOR downstream targets. Whole cell lysates of PC-3 cells treated with ac-LA or lupeol (each 10 μM) for 30 min were analyzed by using Western immunoblotting. Actin served as loading control.(B) Ac-LA rapidly inhibits phorphorylation of PDK1 and Akt, and later also that of the mTOR target p70S6K. Cells were treated and analyzed as in A. (C) Cells were treated as in A for 2 or 4 h, nuclear extracts were isolated and the Akt downstream targets were analyzed by using Western immunoblotting. (D) Cells were treated as in A for 24 h, supernatants were collected, and the VEGF contents were analyzed by ELISA. Chetomin (100 nM) was used as positive control. Results are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, n = 3.