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. 2017 Jul 8;8(33):55147–55161. doi: 10.18632/oncotarget.19101

Figure 5. Ac-LA inhibits cell proliferation in vivo and angiogenesis and induces apoptosis in prostate cancer xenografts.

Figure 5

(A–C) PC-3-xenografts topically treated with ac-LA or lupeol decrease expression of the proliferation antigen Ki-67 and the angiogenesis marker desmin. One day after grafting on CAM of fertilized chick eggs, PC-3 xenografts were treated with either ac-LA or lupeol for 3 consecutive days. On day 5 after grafting, tumors were collected and analyzed immunohistochemically. Ki-67+ cells - brown stained nuclei, desmin - red cellular stain. Data shown are representative of four eggs. Original magnification 200x. (B) Histomorphometric analysis in sections of tumor xenografts shown in (A) Quantification of cell proliferation (Ki-67+ cells/high power field). (C) Histomorphometric analysis in sections of tumor xenografts shown in A. Quantification of apoptosis (caspase 3+ cells/high power field). Results are mean ± SEM, *p < 0.05, ***p < 0.001, n = 4. (D) Inhibition of prostate tumor growth by ac-LA and lupeol (each at 100 μmol/kg) in mice. Animals were treated intraperitoneally starting from day 8 after xenotransplantation. Docetaxel (10 μmol/kg) was used as positive control. (E) Kaplan-Meier graph of mouse survival shows better safety of ac-LA compared to docetaxel and lupeol. Data are mean ± SEM, *p < 0.05, n = 6 mice in each group.