Table 1. Intra-individual comparison of CA4P-induced tumor necrosis (%) among rats with both micro-HCCs and larger HCCs.
| Micro-HCCs | Larger HCCs | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tumor code | CA4P-induced necrosis (%) | Tumor diameter (mm) | *Tumor vascularity | **Tumor differentiation | Tumor code | CA4P-induced necrosis (%) | Tumor diameter (mm) | *Tumor vascularity | **Tumor differentiation | |
| Rat 1 | Tumor 1 | 93.7 | 2.8 | + | I | #107 | 56.2 | 6.9 | + | II |
| #105 | 4.5 | 6.3 | +++ | III | ||||||
| Rat 2 | Tumor 2 | 94.2 | 4.0 | + | I | #012 | 57.4 | 9.0 | ++ | III |
| #007 | 47.5 | 5.9 | + | II | ||||||
| Rat 3 | Tumor 3 | 90.5 | 4.3 | + | II | #166 | 69.4 | 7.6 | ++ | III |
| #164 | 46.2 | 6.9 | ++ | III | ||||||
| #165 | 21.8 | 9.7 | ++ | III | ||||||
| #168 | 16.4 | 6.5 | ++ | III | ||||||
| #160 | 13.1 | 10.2 | +++ | III | ||||||
| #171 | 0.0 | 10.9 | ++ | III | ||||||
| #163 | 0.0 | 5.8 | + | II | ||||||
| Rat 4 | Tumor 4 | 96. 5 | 4.9 | + | II | #080 | 18.8 | 10.3 | ++ | III |
| Rat 5 | Tumor 5 | 82.8 | 3.8 | +++ | III | #056 | 82.0 | 11.7 | ++ | III |
| #054 | 40.1 | 7.4 | ++++ | IV | ||||||
| Rat 6 | Tumor 6 | 23.2 | 4.1 | ++ | III | #049 | 14.1 | 8.9 | ++ | II |
| #044 | 0.0 | 8.0 | ++++ | IV | ||||||
| Rat 7 | Tumor 7 | 1.6 | 4.7 | ++++ | IV | #133 | 79.1 | 9.4 | + | III |
| Mean ± SD | 68.9 ± 39.4 | 4.1 ± 0.7 | / | / | 33.3 ± 28.3 | 8.4 ± 1.8 | / | / | ||
Note:
*Tumor vascularity was semi-quantificationally scored: (+) similar vascular density to the liver parenchyma; (++) dense vasculature without vascular lakes; (+++) denser vasculature with variously sized vascular lakes; (++++) full of enlarged vascular lakes. **Tumor differentiation was graded by a modified 4-scale Edmondson and Steiner system [29]: (I) highly differentiated, consisting of tumor cells of moderate size arranged in thin trabeculae; (II) larger cells with active nuclear mitosis and possible pseudoglandular structures often with steatosis; (III) larger nuclei and more hyperchromatic or increased mitotic figures, granular and acidophilic cytoplasm, often with giant tumor cells; (IV) undifferentiated tumor cells with hyperchromatic nuclei and loss of trabecular pattern often with hypervascularity and angioinvasion.