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. 2017 Jul 15;8(33):55405–55421. doi: 10.18632/oncotarget.19265

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Copyright: © 2017 Guo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A, B) Application of 10 μM DAPT in the osteogenic differentiation medium for 48 h increased mineralization evaluated by alizarin red S staining following 21 days of osteogenic induction. (C) After 3 days of differentiation in osteogenic medium, ALP activity showed an increase in MM-MSCs treated with DAPT. (D) DAPT treatment induced a decreased expression of Hes-1, Hes-5 and Hey-1 genes in impaired-osteo MM-MSCs. (E) The expression of genes, RUNX2, BSP, ALP, OCN, OPN and COL-1 involved in osteogenic differentiation showed higher level in impaired-osteo MM-MSCs cultured in osteogenic induction medium in addition with DAPT for 7 days. (F) DAPT treatment decreased the expression of Hes-1 but increased the expression of RUNX2 in BMMSCs from impaired-osteo MM patients. Each experiment was repeated three times. All values are expressed as means ± SD. *p < 0.05.