Table 1. The clarified, related, and unclear mechanisms of the EBOV lifecycle.

Lifecycle	Clarified and Related Mechanisms	Unclear Mechanisms
Entry	GP-dependent, receptors or co-receptors (e.g., FR-α, integrin ß1, lectins, Tyro3, NCP1) induced endocytosis or micropinocytosis.	Unincorporated entry methods, unidentified proteins or receptors, un-illuminated pathways in different cell lines.
Uncoating and Fusion	GP-mediated, host enzyme cooperation	Unidentified enzymes trigger and accelerate the process.
Transcription and Replication	RNP (i.e., NP, VP30, VP35, L, RNA) complex-mediated.	The molecular mechanism of RNP is unclear. The driving force directing the VP35 peptide to release RNA from RNP is not clear.
Assembly and Budding	GP-tetherin interaction, VP24-induced correct assembly, NP-related nucleocapsid transport and the incorporation into virions, and VP40-inner leaflet association	How does GP antagonise tetherin? How does NP complete its function? How does VP24 contribute to assembly and budding? What is the mechanism used to control VP40 oligomerization? How does myosin-10 influence the localisation of intra-filopodia motility release? How does VP40 associate with the inner leaflet?