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. 2017 Sep 7;7:10895. doi: 10.1038/s41598-017-11327-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiles of CaARF genes in different tissue/organs. (a) Heatmap showing the normalized RNA-seq expression (FPKM) transformed into Z-score. Hierarchical clustering was conducted in R using the pheatmap package with dissimilarity metrics based on Euclidean distance with complete linkage rule. Color key at the bottom represents row wise Z-score. (b) RT–qPCR analysis of CaARF genes in various tissue/stages of development. Expression of germinating seedling (GS) was taken as a reference to determine relative mRNA level in all other tissues for each gene. Error bars indicate standard error (SE) of mean. GS, germinating seedling; ML, mature leaf; YL, young leaf; SAM, shoot apical meristem; FB1–4, stages of flower bud; FL1–5, stages of flower; YP, young pod.