Figure 2.

(A) FXa-mediated calcium responses are inhibited by specific FXa inhibitor Antistasin related peptide at concentrations 25, 50 and 100 μM. (B) Inhibition of FXa-induced Ca²⁺ release by PAR inhibitors and blocking antibodies. (C) Inhibition of thrombin-induced Ca²⁺ release by PAR inhibitors and Antistasin. Data are plotted with mean ± standard error of mean (SEM), 1-way ANOVA **p < 0.05, ***p < 0.001 when compared to FXa alone at all concentrations compared to FXa or thrombin alone. Dotted line represents control buffer. ATAP2: PAR-1 blocking antibody; FXa: Factor Xa; GB83: PAR-2 selective antagonist; RWJ–58259: PAR-1 antagonist; SAM11: PAR-2 blocking antibody, ns: non-significant.