Figure 4.

Effect of drugs on FXa (150 nM)-mediated Ca²⁺ signaling. Cells were treated with HCQ and fluvastatin for 20 hours and stimulated with FXa 150 nM (A) Hydroxychloroquine (HCQ) (used at 1.25, 2.5, 5, 10 μg/ml) *p = 0.02, **p = 0.001, ***p < 0.001 for comparison of FXa stimulation of untreated cells vs 2.5, 5, 10 μg/ml HCQ treated HUVEC respectively. (B) Fluvastatin (used at 1.25, 2.5, 5, 10 μM) ****p < 0.0001 for comparison of FXa stimulation of untreated cells vs 2.5, 5, 10 μM fluvastatin treated HUVEC respectively. (C) Inhibition of IgG-potentiated intracellular Ca²⁺ release in HCQ 5 μg/ml and fluvastatin 10 μM treated cells. IgG that mediated the strongest effect on Ca²⁺ release was selected. FXa + APS IgG vs FXa-only stimulation in untreated cells, *p = 0.03; FXa + APS IgG in untreated cells vs HCQ treated cells, *p = 0.01, vs fluvastatin treated cells **p = 0.009. Plotted with mean ± standard error of mean. Dotted line represents drug alone in graphs A and B.