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. 2017 Sep 7;7:10786. doi: 10.1038/s41598-017-09990-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flocculation level of parental (F6789), haploid (F6789A), transformants (F6789A-Δflo1 F6789A-Δflo5) and V5 strains. V5 is a laboratory haploid yeast derivative from a strain isolated from Champagne. This yeast was previously used as negative control for flocculation (Bidard et al.⁴⁵) and we are sure that it has no adhesive properties. Flocculation was determined in YNB + glucose (2% w/v) (A), in YEPD (B) and in synthetic must (C). (D) Sedimentation without CaCl₂ of non-flocculent strain V5 and F6789A-Δflo5. The data reported are mean values for triplicates. Vertical error bars represent standard deviations.