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. 2017 Sep 7;7:10786. doi: 10.1038/s41598-017-09990-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of cell wall flocculins by FITC-labelled specific Anti-Flop antibody of parental (F6789), haploid (F6789A), F6789A-Δflo1, F6789A-Δflo5 and V5 strains. The S. cerevisiae cells were grown in YNB+glucose. After 48 h (A_{600 nm} = 1), cells were stained with labelled lectin-specific IgGs and examined under a fluorescence microscope (100X oil objective). No fluorescence was observed in V5 strain nor in cells incubated with TBS only.