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. 2017 Sep 7;7:10802. doi: 10.1038/s41598-017-10452-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Shape-based separation of E. gracilis with different aspect ratios due to shape-dependent lateral inertial focusing equilibrium positions. (a) A schematic view of the inertial microfluidic channel and separation principles. (b) A top view (upper) showing the structure and dimensions of the microfluidic device which consists of an inlet, a straight rectangular microchannel, an expansion region, and five outlets with fluidic resistors. Schematics (lower left) and superimposed experimental images (lower right) illustrating the distribution of E. gracilis cells with different ARs at the inlet, downstream, and expansion region. Scale bar = 10 µm.