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. 2017 Sep 7;7:10802. doi: 10.1038/s41598-017-10452-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Shape-based separation of E. gracilis cells with different ARs ranging from 1 to 7 at the outlets at Re = 77. (a) An illustration of five outlets with high fluidic resistance (i.e. serpentine channels). (b) Snap-shot pictures comparing E. gracilis proportion at the inlet and outlets. (c) Superimposed experimental images showing that E. gracilis with different ARs are more likely to exit from different outlets: spherical shaped and long rod-shaped cells exit from outlets closer to the sidewall and centerline, respectively. Scale bar = 40 µm (d) A comparison of purity for E. gracilis cells with different ARs ranging from 1 to 7 at inlet and each outlet. (e) Bar graphs of enrichment factors for E. gracilis cells with different ARs for each outlet. At least 100 cells were measured for inlet and each outlet.