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. 2017 Sep 7;7:10831. doi: 10.1038/s41598-017-11480-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The cell confluence and the output voltage affect the cell-based H₂O₂ generation. (a) A relative small confluence can achieve the maximum cell-based H₂O₂ generation. (b) High output AV voltage significantly weakens the cell-based H₂O₂ generation. In each experiment, a 1 min of CAP treatment was used to treat DMEM (control) or cancer cells (MDA-MB-231 cells, PA-TU-8988T cells, and U87MG cells) immersed in 100 μL of DMEM. The H₂O₂ concentration was measured immediately (about 1 min) after the treatment. Cancer cells were cultured overnight before the treatment. The cell confluence for (b) was 3 × 10⁴ cells/mL. Results are presented as the mean ± s.d. of three independently repeated experiments. Student’s t-test was performed and the significance is indicated as *p < 0.05.