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. 2017 Sep 7;7:10906. doi: 10.1038/s41598-017-10653-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo imaging of the xiphoid injury mouse model. (A) The merged fluorescence signal with a white-light image captured on the fifth day after xiphoid injury. Probe injection was performed at concentration of 0.05 mg/mL 24 hours before imaging (top) and the quantification analysis of signal intensity (bottom). Scale bar: 1.0 cm. (B) Ex vivo imaging of xiphoid tissue (top) and the quantification analysis (bottom). Ex vivo xiphoid samples were isolated from in vivo mouse model after imaging. Scale bar: 2.0 mm. (C) TUNEL staining of xiphoid (left) and the quantification analysis (right) (Arrow heads indicate TUNEL positive cells). Number of TUNEL positive cells was counted and normalized to 1 mm². All the data were calibrated with Student’s t-test (n = 5, *p < 0.05). Scale bar: 100 μm. All the statistical data were presented as mean ± standard deviation.