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. 2017 Sep 7;7:10814. doi: 10.1038/s41598-017-11445-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of PHD2 and HDAC2 interaction by ADI. (a) Induction of HDAC2 and PHD2 interaction by ADI determined by co-IP using anti-HDAC2 antibody followed by Western blotting using anti-PHD2 and anti-HDAC2 antibodies. (b) Reciprocal co-IP using anti-PHD2 in immunoprecipitation and Western blots using anti-PHD2 and anti-HDAC2. (c) Co-IP in 293 cells transfected with recombinants encoding HDAC2-Flag and PHD2-GFP. (d,e) ChIP assays of ASS1 promoter bindings of PHD2 and HDAC2 in PHD2-knockdowned A2058 cells with siRNA (A and B). (f) Effect of HDAC2 knockdown on ASS1 promoter bindings of PHD2 and HDAC2.