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. 2017 Sep 7;7:10902. doi: 10.1038/s41598-017-10787-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative expression analysis of proteome data. (a) qPCR based assessment of gene expression in mock-infected and SpltNPV infected Sf21 cells at 12 h and 72 h post infection. The tested Sf21 genes were reportedly down-regulated at 72 h of infection in proteomics analysis. qPCR analysis confirms down-regulation of 27 out of 28 genes. [tret-1: facilitated trehalose transporter, vATPaseA: V-type proton ATPase catalytic subunit A, NDC2: NADH dehydrogenase [ubiquinone] 1 subunit C2, PABP: poly(A)-binding protein, eIF4A: eukaryotic initiation factor 4 A, PGR1: prostaglandin reductase 1, lark: RNA binding protein Lark, 14-3-3: 14-3-3 epsilon protein, eIF1A: eukaryotic translation initiation factor 1 A, vATPaseH: V-type proton ATPase subunit H isoform 2, eIF2A: eukaryotic translation initiation factor 2 A, chDBP: chromodomain helicase DNA binding protein, MDH: malate dehydrogenase, hag-act: heparan-alpha-glucosaminide N-acetyltransferase, UQCR: ubiquinol-cytochrome c reductase, trx: thioredoxin isoform X1, FOXRED: FAD-dependent oxidoreductase domain-containing protein 1, H2A: H2A histone family member V, NADH-Q7: NADH-ubiquinone oxidoreductase Fe-S protein 7, transaldol: transaldolase, UBE4: ubiquitin conjugation factor E4, MCM7: DNA replication licensing factor MCM7, eIF4E: eukaryotic translation initiation factor 4E, GSTs: glutathione S-transferase sigma 1, COXVa: cytochrome c oxidase subunit Va, ADF1: actin-depolymerizing factor 1, disinteg: disintegrin and metalloproteinase domain-containing protein 12]; (b) Relative expression of four Sf21 genes which were found to be up-regulated in proteomics analysis [pao peptidase: pao retrotransposon peptidase family protein, senp7: sentrin/sumo-specific protease senp7, chitinase: chitinase precursor, ecRb: ecdysis triggering hormone receptor isoform B] The genes show similar mRNA levels in both infected and mock-infected samples; (c) Comparative expression of Sf21 protein Histone H3 in mock-infected and infected cells at 12 h (Lane1, 2) and 72 h (Lane 3, 4) post infection through western blot. Blots depicting expression at 12 h (Lane 1,2) and 72 h (Lane 3, 4) were processed separately. The figure shows no change in expression of Histone H3 at 12 h in mock and infected cells but a marked reduction in its expression at 72 h.p.i in SpltNPV infected cells v/s mock-infected cells.