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. 2017 Sep 7;7:10785. doi: 10.1038/s41598-017-10976-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Diagram of cDNAs corresponding to the two mRNA variants of C. virginica with the position of the indel, and the positions of primers used in this study. (B) PCR products from amplification of the indel using cDNA (left) and genomic DNA (right) as templates (the uncropped images are included in Supplementary Fig. S2). The expected size of the amplicon is 267 bp for the long variant and 97 bp for the short variant.