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. 2017 Sep 7;7:10785. doi: 10.1038/s41598-017-10976-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Expression profile of CvAOXA and CvAOXB in different organs of C. virginica. Relative expression (y-axis) is calculated using 2^−ΔΔCт standardized by the lowest expression of CvAOXB in mantle. Error bars are standard errors. (B) Relative expression of CvAOXA and CvAOXB in gill under air exposure at 25 °C. (C) Relative expression of CvAOXA and CvAOXB in gill under air exposure at 5 °C. Expression levels of control samples (in water) in each time set are used as references. Lines and the secondary axis (right) show the mortality. Letter a designates significant difference between CvAOXA and CvAOXB. Letter b designates significant difference between challenged and control groups. Significance level is set at p < 0.05.