Figure 5.

C33(S) reversed the declining of PLB phosphorylation at Ser-16. (A) Cardiac myocytes were transfected with PDE9A siRNA then treated with PE. ^*P<0.05 vs NC group. ^##P<0.01 vsNC+PE group. n=4. (B) C33(S) (500 nmol/L and 50 nmol/L) and PF-7943 (2 μmol/L), combined with or without PE treatment. ^*P<0.05 vs control. ^#P<0.05, ^##P<0.01 vs PE group. n=4. (C) The phosphorylated PLB was measured in ISO induced heart failure model treated with C33(S). The data were displayed by the ratio of p-PLB to t-PLB. C33-H: C33(S) 9 mg·kg⁻¹·d⁻¹. C33-M: C33(S) 3 mg·kg⁻¹·d⁻¹. C33-L: C33(S) 1 mg·kg⁻¹·d⁻¹). ^**P<0.01 vs control. ^#P<0.05 vs ISO group. n=3–6. Data were expressed as mean±SEM.