Table 2. Graft manipulation strategies and their clinical purposes.

Manipulation strategy	Clinical purpose
Depletion (targeted negative selection)  Pan T-cell depletion   Ex vivo (with or without T-cell add-back)   In vivo serotherapy (ATG or alemtuzumab).  T-cell subset depletion   CD8+ T-cell depletion.   CD3/CD19 cell depletion.   αβ T-cell/CD19 cell depletion.   Naive T-cell depletion. Depletion of immune cells (indirectly)  CD34+ positive selection.	GvHD risk reduction
Inclusion (positive selection/expansion)  Treg cells	GvHD risk reduction
NK cell  γδ T cells	Relapse/infection risk reduction

Abbreviations: ATG=anti-thymocyte globulin; NK=natural killer.