Fig. 3.

Reduced width of proliferative EGL following preterm IVH. a Images of EGL of the developing cerebellum from which quantitative measurements were made of the proliferative width in the respective groups at the time points studied. The image shows the Ki67-positive outer portion of the EGL where proliferation of granule cell precursors occurs and the deeper portion, which hosts the differentiation of granule cell precursors to mature granule cells. Scale bar = 50 μm. b GCP proliferation in the outer portion of the EGL of the developing cerebellum was investigated following IVH by Ki67 staining. Measurement of the width of proliferative EGL was done in cerebellar tissue sections of both sham controls (control, white bars; n at P0 = 6, n at P2 = 6, n at P5 = 5) and IVH pups (dark gray bars; n at P0 = 5, n at P2 = 6, n at P5 = 6) at P0, P2, and P5. Results are presented as box plots displaying medians and 25th and 75th percentiles. Statistical differences between groups for respective time points were analyzed using the Mann–Whitney U test