Table 1. DNA sequence information for GDF9, BMP15, and BMP15R1B missense variants identified in the sheep diversity panel (MSDPv2.4).

Gene	Codon variant a	Position (Oar_v3.1)	Exon	Pro. dom. b	Codon c	Codon allele d	MAF e	Flanking genomic sequence
GDF9	R87H	chr5:41,843,258	1	PRO	c Rc	c Gc = R c Ac = H	0.037	ggtgggagacacagacctggtctcctttcccctctcttagaggttctgtatgatgggcacggggaaccccccaggctgcagccagatgacagagctttgc [R] ctacatgaagaggctctataaggcatacgctaccaaggaggggacccctaaatccaacagacgccacctctacaacactgttcggctcttcaccccctgt
	E241K	chr5:41,841,675	2	PRO	Raa	Gaa = E Aaa = K	0.037	gaaatacaaatggatggagattgatgtgacggctcctcttgagcctctggtggcctcccacaagaggaatattcacatgtctgtaaattttacatgtgcg [R] aagaccagctgcagcatccttcagcgcgggacagcctgtttaacatgactcttctcgtagcgccctcactgcttttgtatctgaacgacacaagtgctca
	V332I	chr5:41,841,402	2	MAT	Rtt	Gtt = V Att = I	0.245	atctgcctaccccgtgggagaagaagctgctgagggtgtaagatcgtcccgtcaccgcagagaccaggagagtgccagctctgaattgaagaagcctctg [R] ttccagcttcagtcaatctgagtgaatacttcaaacagtttctttttccccagaatgaatgtgagctccatgactttagacttagctttagtcagctgaa
	V371M	chr5:41,841,285	2	MAT	Rtg	Gtg = V Atg = M	0.026	tctgagtgaatacttcaaacagtttctttttccccagaatgaatgtgagctccatgactttagacttagctttagtcagctgaagtgggacaactggatt [R] tggccccacacaaatacaaccctcgatactgtaaaggggactgtcccagggcggtcggacatcggtatggctctccggttcacaccatggtgcagaacat
BMP15	L11ΔL f	chrX:50,977,397	1	PRO	indel	cttctt = LL ctt = L	0.344	gtaaaaggaaaggtttaaagcgttatcctttgggcttttatcagaacatgttgctgaacaccaagcttttcaagatggtcctcctgagcatccttagaatc [cttctt/ctt] tggggactggtgctttttatggaacatagggtccaaatgacacaggtagggcagccctctattgcccacctgcctgaggcccctaccttgcccctgattc
	R67Q	chrX:50,977,228	1	PRO	c Rg	c Gg = R c Ag = Q	0.010	cacctgcctgaggcccctaccttgcccctgattcaggagctgctagaagaagcccctggcaagcagcagaggaagccgcgggtcttagggcatcccttac [R] gtatatgctggagctgtaccagcgttcagctgacgcaagtggacaccctagggaaaaccgcaccattggggccaccatggtgaggctggtgaggccgctg
	L252P	chrX:50,971,365	1	MAT	c Yg	c Tg = L c Cg = P	0.083	ttctggtggcatggcacttcatcattggacactgtcttcttgttactgtatttcaatgacactcagagtgttcagaagaccaaacctctccctaaaggcc [Y] gaaagagtttacagaaaaagacccttctcttctcttgaggagggctcgtcaagcaggcagtattgcatcggaagttcctggcccctccagggagcatgat
BMPR1B	M64I	chr6:29,401,381	4	AR	at R	at G = M at A = I	0.026	acacacacacacacacacacacacacacatactttgcctgtttgatctttagcacagatggatattgtttcacgatgatagaagaagatgactctgggat [R] cctgtggtcacttctggatgtctaggactagaaggctcagattttcagtgtcgggtaaggaagataccttggttccactttgtaaccttttattggcaag
	T345N	chr6:29,380,795	9	AS-AL	a Mt	a At = T a Ct = N	0.026	cctgtggtcacttctggatgtctaggactagaaggctcagattttcagtgtcgggtaaggaagataccttggttccactttgtaaccttttattggcaag [M] ctctgtcacttacacactgaaatctttagcactcaaggcaaaccagcaattgcccatcgagatctgaaaagtaagaacatcctggtgaagaaaaatggaa

^aAll variants and sequences are oriented from the sense strand perspective. However, GDF9, BMP15, and BMPR1B are oriented in the opposite direction with regards to the Oar_v3.1 reference assembly. Alphabetical abbreviations for relevant amino acids: E, glutamate; H, histidine; I, isoleucine; K, lysine; L, leucine; M, methionine; N, asparagine; P, proline; Q, glutamine; R, arginine; T, threonine; and V, valine.

^bProtein domain abbreviations: PRO, propeptide; MAT, mature peptide; AR, activin receptor domain; and AS-AL, between the active site proton acceptor and the activation loop domains.

^cIUPAC/IUBMB ambiguity codes used for nucleotides: R = a/g, Y = c/t, M = a/c, K = g/t, S = c/g, W = a/t ³⁵.

^dThe major allele is listed first.

^eMinor allele frequency in MSDPv2.4.

^fThe L11ΔL variant is an abbreviation for p.(Leu10_11delinsLeu), the recommended nomenclature for this variant by the Human Genome Variation Society ³⁶.