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. 2017 Sep 7;50:27. doi: 10.1186/s40659-017-0133-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — miR-22 was downregulated and sirt1 was upregulated in breast cancer cells. qRT-PCR analyses were performed to detect the expression levels of miR-22 (a) and sirt1 mRNA (b) in breast cancer cell lines (MCF-7 and MDA-MB-231) and normal breast epithelial cell line MCF-10A. c, d Western Blot was carried out to determine the protein level of sirt1 in MCF-7, MDA-MB-231 and MCF-10A. *P < 0.05