Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 7;50:27. doi: 10.1186/s40659-017-0133-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — Effect of sirt1 knockdown on tumorigenesis and radiosensitivity of breast cancer cells. MCF-7 and MDA-MB-231 cells were transfected with si-sirt1 or si-NC and cultured for 48 h. Cell viability was detected by CCK-8 assay in transfected MCF-7 (a) and MDA-MB-231 (b) cells. Apoptosis was evaluated by flow cytometry analysis in transfected MCF-7 (c) and MDA-MB-231 (d) cells. Colony formation assay was used to detect survival fraction in transfected MCF-7 (e) and MDA-MB-231 (f) cells exposed to 0, 2, 4, 6, or 8 Gy of irradiation. The expression of γ-H2AX in transfected MCF-7 (g) and MDA-MB-231 (h) cells with 6 Gy radiation was detected by γ-H2AX foci formation assay. *P < 0.05