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. 2017 Jan 21;4(1):4. doi: 10.3390/bioengineering4010004

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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H & E and Masson’s Trichrome Staining for magnetic nanoparticles (MNP) spheroids. Spheroids were fabricated with varying MNP concentrations, type, shape, incorporation method, or no MNP. After (a) Day 3 and (b) Day 40 time points, spheroids were collected, fixed, and processed for histological examination. The H & E stain demonstrates the presence of nuclei throughout all formulations of spheroids after 3 and 40 days. The Masson’s Trichrome stain demonstrates an increased presence of collagen (represented by the blue in the samples) in the Janus, 0.1 mg/mL iron oxide (IO), and magnetoferritin spheroids on Day 40.