Figure 1.

Bioprocess data of the fed-batch fermentation. A fed-batch cultivation of Pseudomonas putida KT2440 was carried out with an exponential feed of octanoic acid (C8), followed by a poly(hydroxyalkanoate) (PHA) depolymerization phase under carbon limitation. The dashed lines indicate A: the time point 12.3 h when the feed changed from exponential to linear mode, B: the time point 16.3 h when the carbon feeding was stopped and an ammonium pulse was added, and C: the time point 24 h when the carbon source was depleted. (a) Optical density (•, OD₆₀₀), volumetric total cell count (♦ vTCC), CO₂ content in the off-gas (--); (b) Carbon (C) feed rate (-), carbon and nitrogen content in the culture supernatant (∇ residual C, ◦ residual N); (c) PHA quantity as determined by gas chromatography (GC) analysis (∎) and by measuring the mean green fluorescence (✴ mean FL1) of BODIPY 493/503 stained cells with flow cytometry (FCM). The Roman numbers (I–X) in proximity of the mean FL1 values mark the FCM samples depicted in Figure 3 in Section 3.3.