Skip to main content
. 2017 Apr 20;4(2):35. doi: 10.3390/bioengineering4020035

Figure 2.

Figure 2

Effect of different media on MiTEC morphology. Aggregates were cultured for 3 weeks in different media: basic medium (BM), BM supplemented with 1×ITS (IM), BM supplemented with 0.1 μM dexamethasone (Dex) and 10 ng/mL transforming growth factor beta 3 (TGFβ3) (BC) and BM supplemented with 1× ITS, 0.1 μM Dex, and 10 ng/mL TGFβ3 (IC). Brightfield images at week 1 (Top Row) show the difference in aggregate size in which the aggregates in the IC medium was the biggest. Alcian Blue staining at week 2 and 3 (Row 2–4) shows maturation of the cartilage extracellular matrix; aggregates in the IC medium were stained most intensely and uniformly. Hypertrophic cells can be found at both week 2 and week 3 in the IM, BC and IC medium.