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. 2017 Apr 20;4(2):35. doi: 10.3390/bioengineering4020035

Figure 4.

Figure 4

Induction of MiTEC mineralization with beta-glycerophosphate. Calcium content, hydroxyproline content and calcium content per µg of hydroxyproline of aggregates cultured in IC medium for 2 or 3 weeks, plus 1 week treatment with beta-glycerophosphate (bGP) (A). Error bars represent the standard deviation (n = 3). (**) denotes p < 0.01, and (***) denotes p < 0.001. Alizarin Red staining of aggregates cultured in IC medium for 2 or 3 weeks, plus 1 week treatment with bGP; red nodules can be seen homogeneously inside the aggregates (B).