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Devitalization and decellularization of MiTEC. Aggregates were cultured for 2 weeks in IC medium, and then were either devitalized by repeated liquid nitrogen (LN2) freeze/thaw or decellularized by SDS. Picture panel shows Alcian Blue staining (Top Row); collagen type 2 staining (Middle Row); and collagen type 10 staining (Bottom Row).
