Figure 2.

Phenotypes of the hot3-2 Mutant and Three Complemented Lines.

(A) Growth of hot3-2 seedlings compared with the wild type after 7 d (left). Seed germination assay in the wild type, hot3-2, and three complemented lines (right). Imbibed seeds were transferred to MS medium, and seed germination was recorded at the indicated time points. Each time point represents the means ± se of three replicates with >100 seeds per replicate.

(B) Phenotype of 10-d-old seedlings growing on vertically placed agar plates. Primary root length and the number of lateral roots were measured. Data are means ± se of three replicates (n = 15), **P < 0.01, compared with wild-type plants (two-tail Student t test).

(C) Morphology of the wild type, hot3-2, and three complemented lines. Two-week-old (top) and 6-week-old (middle) plants growing in soil and the shape of rosette leaves (bottom).

(D) Full-grown siliques. From left to right: the wild type, hot3-2, and three complemented lines (Comp #1, #2, #3). Silique length was measured and data are means ± se (n = 15), **P < 0.01, compared with wild-type plants (two-tail Student’s t test).