Figure 5.

Expression Patterns of Four eIF5B Genes from Arabidopsis.

(A) In silico analysis of the expression of four eIF5B genes. Data were obtained and organized from public microarray data (http://jsp.weigelworld.org/expviz/expviz.jsp) of transcript levels of the four eIF5B genes under heat stress. The black bar underneath the x axis indicates heat treatment period at 38°C, while the gray bar indicates the recovery period at 25°C following a 3-h heat treatment.

(B) Relative expression levels of eIF5B genes under heat stress. RNA from 5-d-old seedlings treated at 38°C in the dark for the indicated times was used for qRT-PCR. The relative levels of eIF5B mRNA were normalized with eIF5B1 set as 1.0. Actin 2 (At3g18780) was used as the internal standard. Data are means ± se from three technical replicates.

(C) Analysis of eIF5Bpro::GUS expression. Different tissue-specific expression patterns of the four eIF5B genes were observed. Bars = 1 mm.