Figure 1.

COI1-Dependent JA Signaling Is Required for Resistance against B. cinerea Infection in Tomato.

(A) and (B) Time course of TD (A) and PR-STH2 (B) expression in response to B. cinerea inoculation.

(C) and (D) Response of wild-type and jai1 plants to B. cinerea infection. Detached leaves from 5-week-old tomato plants were spotted with a 5 μL spore suspension (10⁶ spores/mL). Photographs were taken (C) and the lesion area analyzed (D) at 3 DAI. Error bars represent the sd from three independent experiments (n = 6). Asterisks indicate significant difference from the wild type according to Student’s t test at **P < 0.05.

(E) and (F) B. cinerea-induced expression of JA-mediated defense genes at 24 HAI in wild-type and jai1 plants.

For (A), (B), (E), and (F), 5-week-old plants were spotted with 10 μL spore suspension (10⁶ spores/mL). Leaves were harvested at the indicated time points for RNA extraction and RT-qPCR analysis. Transcript levels of each gene were normalized to ACTIN2 expression. Error bars represent the sd of three biological replicates. Asterisks indicate significant difference from the wild type according to Student’s t test at **P < 0.05.