Figure 9.

TEC1 Is a bHLH Transcription Factor Involved in Auxin-Related Developmental Responses.

(A) Schematic representation of the position of T-DNA insertion in the TEC1 locus.

(B) to (F) Scanning electron microscopy images of wild-type (B), ett-3 (C), tec1 (D), and ett-3 tec1 double mutant ([E] and [F]) gynoecium with excessive production of stigmatic tissue (arrowhead).

(G) GUS staining of pTEC1:TEC1-GUS marker line in inflorescence, shoot apical meristem (inset), at the lower (abaxial) side, and throughout a developing primary branch (arrowhead).

(H) GUS staining of p35S(TEC1-2Intron):GUS in inflorescence and developing branch.

(I) Expression profile of pTEC1:TEC1-GUS in ett-3 mutant background in the gynoecium and at the lower (abaxial) side of a developing primary branch (arrowhead).

(J) Yeast-two-hybrid interaction assay between ETT and TEC1 and respective negative control with empty AD and BD vectors. Interactions are tested on selection media lacking Trp, Leu, His, and adenine (-W-L-H-A) and supplemented with 100 µM IAA.

(K) to (O) Emergence of extra accessory shoots (marked with an asterisk) in the wild type (K), tec1 (L), ett-3 (M), and ett-3 tec1 (N) and its frequency in percentage (O). Statistical analysis is conducted with one-way ANOVA. *P < 0.01

ms, main stem; pb, primary branch; va, valves; sg, stigma; st, style. Bars = 100 µm in (B) to (F), (G) (meristem), and (I) (gynoecium) and 5 mm in (G) and (I) (branches) and (K) to (N).