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. 2017 Aug 30;23:4182–4191. doi: 10.12659/MSM.906389

Figure 2.

Figure 2

Expression of EIF3C in breast cancer cell lines and EIF3C knockdown in MDA-MB-231 by lentivirus-based siRNA strategy. Knockdown of EIF3C expression inhibits proliferation of MDA-MB-231. (A) Efficient knock down of EIF3C at the mRNA level was achieved by lentivirus expressing EIF3C siRNA in MDA-MB-231 and normalized to GAPDH mRNA. Data shown are the mean ±SD of three independent reverse transcript quantitative PCR experiments (** p<0.01). (B) Western blot analysis showed efficient knock down of EIF3C at the protein level achieved by lentivirus expressing EIF3C siRNA in MDA-MB-231, and GAPDH was used as an internal reference. (C) Representative images of MDA-MB-231 cells infected with lentivirus expressing scrambled siRNA or EIF3C siRNA at different time points subsequent to lentivirus transfection. (D) Proliferation of MDA-MB-231 cells was monitored by Cellomics ArrayScan VTI. Proliferation data are shown as cell numbers (** p<0.01). (E) Proliferation data are shown as fold-changes of cell numbers. The results are expressed as the mean ± standard deviation of six separate experiments (** p<0.01).