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. 2017 Aug 30;23:4182–4191. doi: 10.12659/MSM.906389

Figure 3.

Figure 3

Silencing of EIF3C using lentivirus-mediated EIF3C siRNA suppresses colony formation in MDA-MB-231. Downregulation of EIF3C results in cell cycle arrest and induces cell apoptosis in MDA-MB-231. The Scr-siRNA group consists of MDA-MB-231 cells infected with lentivirus expressing scrambled siRNA. EIF3C-siRNA represents MDA-MB-231 cells infected with lentivirus expressing EIF3C siRNA. (A) Representative images of colony formation of MDA-MB-231 cells in six-well plates. The Scr-siRNA group consists of the MDA-MB-231 cells infected with lentivirus expressing scrambled siRNA. The EIF3C-siRNA groups consist of the MDA-MB-231 cells infected with lentivirus expressing EIF3C siRNA. (B) Colony formation of MDA-MB-231 cells treated with EIF3C siRNA. The results are expressed as the mean ± standard deviation of three separate experiments (* p<0.01). (C) Cell apoptosis by Annexin-V staining was analyzed with FACS. (D) Cell apoptosis in cells treated with EIF3C siRNA. The graph expresses the mean ± standard deviation of cell percentage in apoptosis from three separate experiments (** p<0.01).