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. 2017 Sep 5;5:e3754. doi: 10.7717/peerj.3754

Figure 2. HSE and MSE preparation.

Figure 2

(A) Time frame for cell culture and DED preparation to construct HSE and MSE models. (B) Time intervals at which the HSE and MSE models are cultured and inspected. (C) Schematic of the circular barrier assay showing how cells are placed inside the barrier on a DED within a 24-well tissue culture plate. (D) DED with cells submerged in full Green’s medium. (E)–(F) Schematic and image of the HSE and/or MSE models lifted to the air-liquid interface on a sterile stainless steel grid with full Green’s medium placed in a 6-well plate. Scale in (F) bar corresponds to 6 mm.