Table 2. Primary antibody protocols.

Details of the primary antibodies and the antigen retrieval method used to detect the basement membrane (Col IV); terminal epidermal differentiation (Loricrin); migration (Vimentin); proliferation (Ki-67); and invasion (S100).

Antibody	Primary antibody			Antigen retrieval method
	Antibody type	Source	Dilution	Time and temperature	Buffer	Time and temperature
Collagen IV (Col IV)	Mouse	DKSH, Australia	1:50	1 h, 37°C	Sodium Citrate (pH6.0)	20 min, 80°C
Ki-67	Mouse	Sigma Aldrich, Australia	1:100	1 h, 37°C	EDTA (pH8.0)	30 min, 90°C
Loricrin	Rabbit	Dako, Australia	1:100	1 h, 37°C	EDTA (pH8.0)	5 min, 97°C
S100	Rabbit	Dako, Australia	1:3,000	1 h, 37°C	Sodium Citrate (pH6.0)	5 min, 95°C
Vimentin	Rabbit	Thermo Scientific, Australia	1:800	12 –24 h,4°C	Sodium Citrate (pH8.0)	20 min, 80°C