Table 1.

Primers used for qPCR. SYBR green was incorporated into qPCR reactions with the primers listed below, using cDNA prepared from PMN samples as described in the text

Transcript	Forward primer	Reverse primer
ARG-1	5′-AAGCAGACCAGCCTTTCTCA-3′	5′-GCCAAGTCCAGAACCATAGG-3′
CTLA4	5′-TGGAGATGCATACTCACACACA-3′	5′-TTCATCCCTGTCTTCTGCAA-3′
CXCL8	5′-CTGGCCGTGGCTCTCTTG-3′	5′-CCTTGGCAAAACTGCACCTT-3′
IL1-β	5′-ACGAATCTCCGACCACCACT-3′	5′-CCATGGCCACAACAACTGAC-3′
IL10	5′-GGTGATGCCCCAAGCTGA-3′	5′-TCCCCCAGGGAGTTCACA-3′
IL12p40	5′-CGGTCATCTGCCGCAAA-3′	5′-CAAGATGAGCTATAGTAGCGGTCCT-3′
IFN-γ	5′-CCAACGCAAAGCAATACATGA-3′	5′-CGCTTCCCTGTTTTAGCTGC-3′
β-Defensin	5′-GCAGTGGAGGGCAATGTCTC-3′	5′-TTCCCTCTGTAACAGGTGCCTT-3′
β2-MG	5′-CTCCGTGGCCTTAGCTGTG-3′	5′-TTTGGAGTACGCTGGATAGCCT-3′
CXCL9	5′-TGCAAGGAACCCCAGTAGTGA-3′	5′-GGTGGATAGTCCCTTGGTTGG-3′
CXCL10	5′-TGAAATTATTCCTGCAAGCCAA-3′	5′-CAGACATCTCTTCTCCCCTTCTTT-3′
CXCR1	5′-AGG GGC CAC ACC AAC CTT CTG-3′	5′-AGT GCC TGC CTC AAT GTC TCC A-3′
CCL4/MIP-1	5′-CTGCTCTCCAGCGCTCTCA-3′	5′-GTAAGAAAAGCAGCAGGCGG-3′
CCL5/RANTES	5′-GACACCACACCCTGCTGCT-3′	5′-TACTCCTTGATGTGGGCACG-3′

IFN = interferon; IL = interleukin; PMN = polymorphonuclear leukocyte (neutrophil); qPCR = quantitative polymerase chain reaction.