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Primers (forward [F] and reverse [R]) and probes (P) used to assess gametocytemia by qPCR
| Gene | Sequence (5′-3′) | Product size (pb) | GeneBank accession no. |
|---|---|---|---|
| pfs16 | F: AGTTCTTCAGGTGCCTCTCTTCA | 111 | M64705 |
| R: AGCTAGCTGAGTTTCTAAAGGCA | |||
| P: dFAM-TCTCAAGGTCTTTCTGGAAAAGATTCTGCTGACAA-BHQ-1 | |||
| pfs25 | F: AGACTGTAAATAAACCATGTGGAGA | 156 | AE014185.2 |
| R: ACCGTTACCACAAGTTACATTCT | |||
| P: dFAM-TGGAAATCCCGTTTCATACGCTTGTAAATGTAATC-BHQ-1 |
qPCR = quantitative polymerase chain reaction.
