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. Author manuscript; available in PMC: 2017 Sep 8.
Published in final edited form as: Biochemistry. 2015 Aug 21;54(35):5414–5424. doi: 10.1021/acs.biochem.5b00759

Figure 2.

Figure 2

Time course of autoproteolysis of PRPR20-123 and PR20PR-123. Hydrolysis of the chromogenic substrate in 50 mM sodium acetate (pH 5.0) containing 250 mM sodium chloride as a function of time of incubation of (A) PRPR20-123 and (B) PR20PR-123 at room temperature (in hours, prior to assaying). Aliquots of (C) PRPR20-123 and (D) PR20PR-123 were withdrawn at the indicated times, mixed with gel loading buffer, subjected to SDS-PAGE, and stained. For comparison with parent constructs, PR and PR20, see Figure S1.