Fig 4. CRP treatment exacerbates oxidative stress ex vivo and in vitro.

Primary cultured cells (A-C) and OKP cells (D-F) were treated with or without CRP in the presence of H₂O₂ (200 μM for 24 hours) or vehicle (PBS). (A, D) LDH release in primary cultured tubular cells or OKP cells with or without CRP treatment at baseline and oxidative stress. (B, E) 8-OHdG release from primary cultured tubular cells or OKP cells with or without CRP treatment at baseline and oxidative stress. (C, F) NGAL protein expression in the primary cultured tubular cells or OKP cells with or without CRP treatment at prior and post oxidative stress. Data are expressed as means ± SD of at least 3 independent experiments for each group and statistical significance was assessed by one-way ANOVA followed by Newman-Keuls test. *: P<0.05, **: P<0.01, ***: P<0.0001 between two groups.