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. 2017 Sep 8;12(9):e0181848. doi: 10.1371/journal.pone.0181848

Fig 5. CRP impairs autophagy in vivo.

Fig 5

(A) LC3 II/I and p62 levels in PLC3 mice and LC3 mice prior and post-IRI by immunoblotting. (B) GFP-LC3 punctae in PLC3 mice and LC3 mice prior and post-IRI by immunohistochemistry. (C) RFP-LC3 and GFP-LC3 punctae in RG-LC3 mice and PRG-LC3 mice prior and post-IRI by immunohistochemistry. (D) Representative TEM for autophagosomes and autolysosomes in the kidneys. Data are expressed as means ± SD of at least 4 mice from each group and statistical significance was assessed by one-way ANOVA followed by Newman-Keuls test. *: P<0.05, **: P<0.01, ***: P<0.0001 between two groups. A: autophagy; AL: autolysosome; and L: Lysosome.