Fig 1. Behavioral tests showing significant differences due to Slc1a1-genotype.
Behavioral data were analyzed for significance using a General Linear Mixed Model ANOVA incorporating two fixed factors (Genotype, Sex) and one random factor (litter Cohort) followed by a with a Dunnett’s post-test. There was no significant difference (nsd) in Elevated Plus Maze (EPM) performance, Total Distance Traveled in the Open Field (OF), Novel Object Recognition (NOR) performance, or Acoustic Startle Habituation (panels A, C, G, I), but 5 behaviors including Time Spent in the Center vs Edge of the OF, Y-Maze performance, Prepulse Inhibition (PPI), and overall object interaction time in the NOR (panels B, D, E, F, H) showed significant differences between the HET and WT mice (* = p < 0.05) and were also significantly changed or trending toward a similar change in comparisons of KO and WT mice. A trend was defined as a change in the same direction as the HET mice that exceeded the 95th CL of the WT mean. Since there were no significant Sex x Genotype interaction effects and no main effect of Sex, histograms only indicate Genotype. Data are shown as mean ± SEM. WT, wildtype; HET, Slc1a1-heterozygous; MUT, Slc1a1-KO; PPI, prepulse inhibition.