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. 2017 Sep 8;12(9):e0183854. doi: 10.1371/journal.pone.0183854

Fig 8. Confirmation of SLC1A1 expression changes in transfected differentiated SK-N-SH cells.

Fig 8

Using real-time RT-qPCR, we confirmed that in SK-N-SH cells treated with SLC1A1 shRNA or cDNA-containing plasmids, the expression level of this gene significantly decreased or increased, respectively. When normalized to the SLC1A1 expression level in the non-target shRNA treated SK-N-SH cells (Control), the cocktail of three validated human MISSION SLC1A1 shRNAs had knocked down the SLC1A1 mRNA expression by 53%. Conversely, SLC1A1 cDNA had increased the mRNA expression level of this gene by 2.4-fold.