Skip to main content
. 2017 Sep 8;12(9):e0183854. doi: 10.1371/journal.pone.0183854

Fig 9. SLC1A1 knockdown or overexpression affects the neural cytokine release following oxidative or inflammatory challenge.

Fig 9

The differentiated SK-N-SH cells subjected to overexpression or knockdown of SLC1A1 were treated with either LPS (100ng/mL) or rotenone (100nM) or 0.01% DMSO as the vehicle. Using a cytokine profiling assay, the levels of different cytokines in the supernatants of each transfection/ treatment condition were measured. The cytokine results are displayed after hierarchical clustering. Two-way ANOVA indicated a significant effect of SLC1A1 expression level (shRNA or cDNA plasmid constructs) and treatment (LPS, RO or vehicle) on all but one cytokine (IL-10) and a significant effect of their interaction on several of the cytokines studied (GM-CSF, TNFalpha, MIP-3alpha, IL-4, IL-6, IL-21). Ctrl, control (non-target shRNA transfection); RO, rotenone; LPS, lipopolysaccharide.