Fig 2. Transmission electron microscopy of Brevundimonas species.

Brevundimonas species were cultured in PYE medium to mid-exponential growth phase and imaged by TEM. Cells were counted to determine the percentage of cells displaying polar extensions (% stalked). Between 92 and 248 cells were counted for each species. With some exceptions, percentages of stalked cells as determined by TEM were close to the number derived by phase contrast microscopy, supporting the results that these organisms produce stalks relatively rarely under nutrient rich conditions.