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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Anal Chem. 2017 Aug 2;89(16):8244–8250. doi: 10.1021/acs.analchem.7b00357

Figure 5.

Figure 5

Comparative evaluation of different sample treatments for bottom-up proteomics. After tryptic digestion of a yeast protein extract (Promega) using different three methods independently, the digested peptides were subjected to LC-MS/MS analysis. For protein identification, MS/MS spectra were searched against the UniProt yeast proteome database by ProteinPilot using the following parameters: cys alkylation, iodoacetamide; digestion, trypsin; processing parameters, biological modification; and search effort, through ID. The values represent the averages from three independent experiments ± S.D.