Skip to main content
. 2017 Sep 8;12(9):e0184308. doi: 10.1371/journal.pone.0184308

Fig 3. Sequence analysis of CLICs.

Fig 3

(A): A putative NLS sequence is identified in EXL-1 protein sequence, but not in EXC-4. All human CLICs have a NLS motif except CLIC3 (shown here CLIC2 and CLIC4). EXL-1, CLIC2, and CLIC4 share conserved residues Lys at P2 (second binding position to importin-α) and Lys/Arg at P5. Both EXC-4 and CLIC3 sequences, however, lack NLS. Conserved amino acids are in red. NLS from CLIC are underlined. Two amino acids at P2 and P5 are bolded (B): Phylogenetic relationships among animal CLIC proteins. The tree was built using the maximum likelihood method. Nodes with higher than 70% bootstrap support are indicated with a star.